Transactions on Mass-Data Analysis of Images and Signals (ISSN:1868-6451)
Volume 1 - Number 2 - September 2009 - Pages 210-223
Device and Method for Acquisition and Automatic Data Processing in Virology
L. Ichim1,2 and R. Dobrescu1
1 Department of Automatic and Industrial Informatics, Faculty of Automatic Control and Computers, Politehnica University of Bucharest,
313 Spl. Independentei, Bucharest, Romania
2 Department of Bioinformatics and Hardware, “Stefan S. Nicolau” Institute of Virology, Romanian Academy,
285 Sos. Mihai Bravu, Bucharest, Romania
Abstract
This paper proposes to use Mie light scattering measured by fibre spectrophotometer mounted on a microscope to detect the difference between infected cells and normal cells. It goes through the theory of light losses at each interface within the assembly evaluating the difference between air optics and oil immersion optics arriving at some equations describing the expected outcome. Cell nuclei size distribution was determined measuring Mie scattering spectra registered using an original optical device. The spectra collected are analyzed by fractal dimension analysis according to Hurst method and Height-height correlation method. The experimental data are presented showing some differences between averages and standard deviations for two kinds of samples. The experimental device based on optical methods allowed to evidence cellular changes for samples analyzed using Mie scattering spectra. The results show that fractal dimension may be a useful tool to clearly discriminate between infected cells and normal cells.